ImageJ (Fiji) as a free, useful tool for medical researchers and students; A review article

Abstract

Digital image processing is increasingly being used in a variety of industries, including food processing, medical science, particle technology, cement, and powder manufacturing. Medical image processing is a discipline in medical science that involves the use of technology to take images of the human body's interior in the least invasive way possible. As medical and biological sciences advance, imaging has become a more important discipline. One of the most useful programs is ImageJ, a public-domain Java image processing program inspired by NIH Image for the Macintosh. In this study, we demonstrated some, but not all, applications of ImageJ in the medical field and for medical and biological students that can be easily implemented in their institutions.  One of these applications was bacterial cell counting, in which a microscopic image of

 gram-stained bacterial cells was captured using a student's smartphone, treated with ImageJ, and the bacterial cells were easily counted automatically using ImageJ. The second application of ImageJ in this review was to calculate the antimicrobial zone of inhibition. We calculated the percentage of the inhibition zone for three different amoxicillin antibiotic brands using very simple steps. The third application of ImageJ was to analyze a CT scan brain images, and we were able to define the hemorrhage location. Finally, we demonstrated that this free software can estimate protein-protein colocalization. This technique is useful in many cell biological and physiological studies to demonstrate the relationship between pairs of biomolecules. In another example of co-localization, the researchers confirmed the SyGCaM2-mCherry sensor's presynaptic localization to hippocampal synapses, where it was co-localized with a bassoon (a presynaptic protein) in the stratum radiatum of area CA1. In general, ImageJ is a very useful, free program that can be used easily by specialized people and the beginner medical students.